Wiki source code of ILEWG EuroMoonMars
Last modified by Barry Bishop on 2016/10/09 15:28
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1.1 | 1 | **Synopsis:** Exp. A: Biomedical assessment, obtaining saliva samples from the suit tester; Exp. B: Sterile collection of soil/ice samples for PCR and phylogenetic analysis |
| 2 | **Institution (PI):** Exp. A: Rai Balwant , Vrije Universiteit Amsterdam & JBR; Exp. B: Luisa Rodrigues, Vrije Universiteit Amsterdam & Aveiro Univ. | ||
| 3 | **Responsible on-site:** Exp. A: Rai Balwant; Exp. B: Luisa Rodrigues | ||
| 4 | |||
| 5 | === Description === | ||
| 6 | |||
| 7 | __Experiment A / Biomedical assessment__ | ||
| 8 | Background: Human performance is affected by physiological and psychological factors which can critically affect mission outcome in both spaceflight and other extreme environments. | ||
| 9 | Materials and Methods: | ||
| 10 | Saliva experiments: Saliva samples will be taken with specialized saliva collection device from | ||
| 11 | each crew members before and after tasks. Saliva will be preserved for further analysis. | ||
| 12 | Vital signs. : Heart rate, heart rate variability, pulse rate, blood pressure and respiration rate, Vo2 max will be measured before and after tasks. | ||
| 13 | Effect of greenish blue light: Saliva samples and vital signs will be taken before and after 5 minutes exposure of light. | ||
| 14 | List of instruments: | ||
| 15 | * Gloves | ||
| 16 | * Vital sign. measuring sensors | ||
| 17 | * Greenish blue light | ||
| 18 | * Salivary samples collection device and storing tubes. | ||
| 19 | * Standardized (medical analysis) software | ||
| 20 | |||
| 21 | __Experiment B / Sterile Soil Sampling__ | ||
| 22 | DNA extracted from ice will be analysed using PCR amplification of both Bacterial, Archaeal 16S- rRNA genes, as well Eukarya (Fungi in particular) 18S-rRNA genes, using specific primers. | ||
| 23 | METHODS | ||
| 24 | The study comprises: | ||
| 25 | |||
| 26 | 1. ice sampling (about 10 cm depth) of various age (if possible) and chemical composition, in | ||
| 27 | sterile conditions | ||
| 28 | 1. DNA extraction from liquid water melted from ice, | ||
| 29 | 1. PCR amplification of 16S-rRNA and 18S-rRNA genes and gene library construction, | ||
| 30 | 1. eventually, sequencing and phylogenetic analysis of genes of some samples. | ||
| 31 | |||
| 32 | During ice sampling it will be taking in consideration the potential microbiological contamination assessment. Below is presented the sampling procedure that will be tested on ice cave. | ||
| 33 | |||
| 34 | === Experiment Data === | ||
| 35 | |||
| 36 | |=Directories/files|=Description | ||
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3.1 | 37 | |[[27-Apr-2012 to 02-May-2012>>url:https://mission.oewf.org/archive_downloads/1/10-ILEWG/sent%20to%20data%20archive%20Reconnaissance%20pics%20&%20Samples%20EuroMoonMars%20Dachstein%2027%20April%202%20May%202012.xls]]| reconnaissance data spreadsheet |
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1.1 | 38 | |
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2.1 | 39 |